Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIKLGKKISEGKTVDVYESGD----LAVKVFKPD--APKTVVFYEAFMDSKVEETGLNVPKIKEVELIDGKWAIATELIKGKTLAQIMQEEPENCDGYLDDMVELQLSIHAKKVTGIS------KLK----DKLREEIESLD---VI---DHIKRYDLLTRLDS--TPKHVKLCHGNFGPENIVVTDDNKVYIVDWIGASAGNASADVAKTYLKLALTS--TETAEKYLNLFCKKTNTAKTYVQEWLPIMAASTLAKGVTSKEEKDLLFTWLDVVDYS 3TM0 Chain:A ((15-246)) --KYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG-KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED-EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI--------------------------------------------

General information: TITO was launched using: RESULT: Template: 3TM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 887 59283 66.84 287.78 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 66.84 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_3TM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tm0-query.scw PDB file : Tito_Scwrl_3TM0.pdb: