Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNRVSSALEDAFPNRTVDNVSSAGISWNAQNRTVAVDFGDGQRVFLKVAVNGD-RSRIERERAVISYVGANCAVSVPNVIATETTG-PVPY----LATEPVSGQPLTTLLADATRTERAALAHDVGSALARVHDCRFEEHGHVVGGDADHLELDTGTWTDVLVETISEMREMAPCDRFDQHFEMVSDAVERNRDRLDRASATLLHGDPACPNCFRTG-D-GVGFLDWELSHVGDPVRDRSRTLDQQFDSLREGSPEGVVSAFYDGYRSQAGGLPSGFEERRPIYEVIRLLSVSGYFERTADYRDESRSDLAAWLEAEMRRRLDAIRR 5IGI Chain:A ((10-256)) --SQLYALAAR-HGLKLHGPLTVN--ELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV---VPQDSEVFAESFATALAALHAVPISAAVDAGM-----LIRTPTQARQKVADDVDRVRREF--VVNDKRLHRWQRWLDDDSSW--PDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF--------GEEGLAKLLLTYEAAGG--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 996 -16156 -16.22 -67.88 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -16.22 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: