Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEVREYGKKSQPKIRYSQFDAEDIRKELLERYGME-MPVECRLFDYG-MNDIYRIKTKKETYFLRISLAGLH-TKKDY-EEEMHILNILIQNGIKAAAPVKMRGMPGRQEDYVWELHAPEGIRYAMLFTEAPNSPS---DDKESSACNLGSMLAGIHNISDREKFQVSRP-PID---MCQLVEKPLSNIRPF-LA---DREQDFSFLKESAEKLAGYIKGKLPETAPVYGFCHGDVHSGNVFFA-G--DKPTLFDFDCMGYGWRSYDICVYAWNETFLD--------ENYIEGSVWKKYLEGYES--VRPLSAEEKETIPAFAALRQLWLMGLHADVMDRNAGCCWYQDDYFDSQIKMFRLWLDRCSL 4OCV Chain:A ((24-322)) ---------------------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIV-----RTTSGDTWAEI----DGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDG-LRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTME---------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1147 9774 8.52 36.07 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 8.52 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: