Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MEPSFENNLATVIARRFEGAKLTSVNQLTAGASQQTFRLTVEEGSGETSVYAFRRAQP---------------GLESSSYGQLPPSLEVELLQLAAEGNVPVP---EIHCVVG----------AEDGLGD-GYIMEWLEGETMGQRIIKLPELADARESLAFEC-GQ------ALARIHALPVSRSLSEKLHSVSPEALVRETWDAYKALDTPQSMIDYTAQWLLSHLPQDSRTTLVHGDFRNGNLMVT--PDGIKAVLDWELCHIGDPMRD-------LGWLCVN--------------------SWRFGNRSLPVGGFGRVEELVAGYESVSSHRVDRDALR-FWEVFG---------SF-WWS-ITTLGMAKTWRTGETPSVERPVIGRRSTEAQMDCVNLLIPGK------VTSLIEAVEDQNLPSASELINSVQSHLREHVAEKLEGADKFLV---RVAINSLSIADRELAYRDQAERAERVGLSALLSDPENADRPFAADAA-DMCGKADTLADLRW-R-LVTALREHKPLNAKILEEHLRQTVAHRLFIDQPKYSALID 3LMM Chain:B ((9-574)) EGRREQLIAQVESILASAADGRVQKTKETQSVDFKEEAGRRN-GPQIEPGKPENPEAADKLADEVACMANTPGGGALIVGIEDKTGRIIGTELDIDWLRQGIFTRIDVAPDVVAKRVLGQRVLAIYVAAAAEPIEDTSDRLRWRVGDS-CRPVDRAEWWEYQRAQSGFDPMAQVTTATLGDARPAALALARKWDPAFAELTDEELLRG----IGALD-AEGFLSQAGKLLFTSLDRTA-IELSIFDVHGGQVLNRVVPEPEKSCLE-QLDYLEQALNVVNKNVPEIPRLAVREAMLNAMIHRDWNRSEPIDVRWIELDSTLIVRSPGGFPAAITSENVLSNRAARYPALADLYRALGLVDKQGVGVDRMYQAMIALGHRPPTIEEIAGPFVETTLVGGRPVLPVLELVSSIVPEARQDDYRIAIVLYLLFQRPFITIDVVARGLQSG-KEAARNALEAARQTTVAGAPLIIAHDGVWLLGNACREILRKVEPSPFSPV--RYLSTDQAELTNAAMLWLSEVGDLATSDLMAMCGVSRGTAKACVDGLVDEERVVAVGGG---RSRRYRLV--

General information: TITO was launched using: RESULT: Template: 3LMM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2439 65565 26.88 142.53 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 26.88 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.161

(partial model without unconserved sides chains): PDB file : Tito_3LMM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3lmm-query.scw PDB file : Tito_Scwrl_3LMM.pdb: