Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKLYEHDITAIAWQDKHELLERILQQAG-TIAITPLMQGMEAEAVKVATDADRYVLKIWSRDSK--PDIRLQYQVMNMLHERGISVSKPIGWGINASGHSVLLTTFDGD-PLRDG-NAHTMREIANLLDHIHRISPEEIDR--H-TRLPRY-NFRDYFFPGMHEYPDLYPLLEQLVELAS--PTHKRIIHGDFHLGNIVEQQ-GRLTVIDWTNVQLGDPRYDFAWSLTLQQFYIPEHFAEVFRDTYVAMNEIQREELEVFQALACLRWMLLYRNGGVPMGADTLERVRRLLTSNPHLHGVTI 4R7B Chain:A ((28-274)) --------------------KISSLLSQEEEVLSVEQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDI---EAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPY-ANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLES--EFTSQEEETFLSHYESDQTP--VSHEKIAIYKILQDTIWSLW----------------------------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1004 36104 35.96 153.63 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 35.96 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: