Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASDRQDLSDRIARAIGHGVRRLSPLSGGCVGDVLRAELEDGAAPVVVKVADDAGGGLDIEGWMLRYLADTS-RLPVPG--------VLHAEAGLLVMEEIQAGGGGISASCQEHAA-DLLADLHSVTWD---AFGLERDTLIGGLHQPNPREASW-RTFFR-----DHRLLYMADESHRAGRLPADVRRKVDTLAGRLDDWIEEPDAP-ALIHGDMWTGNVLCDGGRIAGFIDPAIYYGDAEIELAFS-TL--------FGTFGEPF-FRRYQEKRPLRPGFFEARRDL-YNL-YPLLVHVRLFGGSYVGSVSRTLDRFVG---------------- 2Q83 Chain:A ((11-346)) LSAEDAKKLTELAENVLQGWDVQAEKIDVIQALVWKVHTDSG--AVCLKRIHRPEKKALFSIFAQDYLAKKGMNVPGILPNKKGSLYSKHGSFLFVVYDWIEGRPFELTVKQDLEFIMKGLADFHTASVGYQPPNGVPIFTKLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRAPLT----EEQKQVMFIDMLFPYELYDVIREKYVRKSALPKEELESAFEYERIKANALRQLI

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1127 -18962 -16.83 -66.77 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -16.83 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: