Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFARDALSTAVETACDPDPAVDTISTIEAGNN-HTYRVTLADGTDLFLKVGTRF--PDAFPAEPATMRRVRAETDLPVPRVHGTGTDPL--EYPYAVYEYVPDCEAEWAG--DLSHEAAERLCREAGRHLCELHQITFPQFGRIGLDTDASADDTLVADAPDADALTVVDPTPFDDLLRQSLDRQVTELADSPFDTRRDSLEAFGTDLIDRSDFADVTPALVHGDYRLGNLCVDPSAEQITAAVLDWELPTALDPLWDAVMAQSLLTAGHRLDPDSRQTLRRAFWEAYGTSTAD---TPRRRCYELLARIRLARHLDTECRGLSEAARRARITDHHEAFDALLVDR 3N4U Chain:A ((3-275)) -TYTFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEI----NRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLD---------------------FREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEI----YFKYYPCLIHNDFSSDHILFDTE-KNTICGIIDFGDAAISDPDNDFISLMEDDEE--Y-----GMEFVSKILNHY-KHKDIPTVLEKYRMKEKYWSFEK----------------------------------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1144 24959 21.82 94.90 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 21.82 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: