Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVPLSIHTTSYSMAAWDITTADEAWLIELCHKSADEGRQIGGQEHGGPKVVRIS-----DRIVAKYG-NIRR-----------SELAAQEVAYHNTDRSIVHIPKVHRFFESDGQSYLFMEYVEGQTLEDVDFE-------THKDIPIRVANILAHLQQILGDCDSPGPVTGGEAHGYIFGDEGAGTAFDSIEDMNVYMNKRLDKMNEYLSRQEDQRRFDHLDLTPYALVLCHGDICRRNIIFESDGSLCLVDWGFAGFYPRIFELAAISYVVQNNAAFKDPIIHEFT-KLLSLTDKEKQDIDRFRAVRFANLRWSFRDRRTTAEEDKFIQELYETQKRIKE-EQETAGIQSFIISELLDGLL--EQKSRPDLEVRHVPRPWTGSQTAIGHVKRVVVVGLDVNVASFILAMKILSSRRIFVIRTTQGSPK 2Y7J Chain:A ((102-364)) ------------------------------------------IGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVA-GHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFL---------------------------------------------------------------------HANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLREL-CGTP---GYLAPEILKCSMDETHPGYGKEVDLWACGV-----ILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQA-LQHPFFE----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Y7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1019 -10959 -10.75 -46.63 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -10.75 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_2Y7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2y7j-query.scw PDB file : Tito_Scwrl_2Y7J.pdb: