Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVYTQLTDDQFSAFCQRFGVSFACAIPITQGIKNSNWFIQTTDDVDGAHSYVFT-LFEERPLEDIEKMAVILNQLDGKLPVAAPLSLVDSAEKCYVIRYDNKAITLVPCLAGSHPQQ-TTQAMCHEIGTALAMLHETLQALQP---SEQYGV-PLYPWSDVRDRERQFMPADEAKLMSDIWQSYTDLPLATLPK-GLCHLDMFADNTLWNLSLNNSQKGEESLTGLLDFTEVSVEHYVMDIAITINDFCTTWGDAEQGESVNFDRSKMAAF-LQGYESKRSLGADEKRALPVMLAKAAVIF-WLLRLNVIHYNRTEGRTGDNIMVKNPDLMKRLAAYHWSHVEKAKNTVFVLLDNLLASQKDIQIVGVFSNITQAEQARDKLSNSSEFAIKEYTVDQLS 3CSV Chain:A ((4-333)) ------SREDEIRDFLATHGYA--------DWNRTPRYQRLRSPT----GAKAVLMDWSPEEGGDTQPFVDLAQYLRNL-DISAPEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMP---LYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLE--GDMVFVHRDFHAQNLLWL-----PEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDV------------PAQVEAQMIDHYIQAT--GVDESHFRSAYAVIAVQRNMRILGIFARLSQRF----------GKRHYIEFVPRVWAHFERGLAHPALASAAEEILNALP----------APAPEVLERLRA---------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1503 13654 9.08 43.62 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 9.08 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: