Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGGNMAEKDLATRVARV--------LGRNSVRLEDLPPGSSRGAARVLSEE-GEPIAFLRIDLGVATDVTVGKALVSEMALVKRAHDLGFPVPKVFALLNDPPVAVMELAAGTARPDEAEAERVGPEFMGHIARLHSIAPAEFGLDAPATVSDAIRQDLDCYMADAEARGITRRPLVRLAARILRHALPRSAEPPAMLHGDVGAGNFMVDNGRVSAIL--DWELAHAGDIHEDLAWLWTRGAHTAFGDPRQRIAEYEAAAGKALDPDRLAWHVAFVTFKSVIGLQRRMYAQGDDPGLLIILIAILTYETLLCAALGRILGIQLDMMEEAPVEGVTETVRLIELMRHVAPPANREGELLQQYLRAEAAQRPWNADRLRADARERLGAHPDQLDMLVDSAAPDRFADLLRVFGAAAGRRCKAFPNAERRVRRALAIGLGKQN 1KOB Chain:A ((23-374)) INDYDKFYEDIWKKYVPQPVEVKQGSVYDYYDILEELGSGAFGVVHRCVEKATGRVFVAKFIN---TPYPLDKYTVKNEISIMNQLHHPKLINLHDAFEDKYEMVLILEFLSG-------------GELFDRIA--------------------------------AEDYKMSEAEVINYMRQACEGL--KHMHEHSIVHLDIKPENIMCETKKASSVKIIDFGLATKLNPDEIVKVTTATAEFAA----------PEIVDREPVGFYTDMWAIGVLGYVLLSGLS---PFAGEDDLETLQNVKRCDWE-----------------FDEDAFSSVSPEAK--DFIKNLLQKEPRKRLTVHDALEHPWLKGDHSNLTSRIPSSRYNKIRQKIKEKYADWPAPQPAIGRIANFSSLRKHRPQEYQIYDSYFDRKEA-------

General information: TITO was launched using: RESULT: Template: 1KOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1807 50877 28.16 149.20 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 28.16 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_1KOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1kob-query.scw PDB file : Tito_Scwrl_1KOB.pdb: