Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAANPPRPSR--SLQGKVAIVTGAGSRGETIGNGRAIALLLAEDGAKV-VCVDREEALAQRTAELVLAENEGASALAVAADVSSAEECARVVDAALRLFGRVDVLVNGVGVLGPPGTAVDVDAARWARGLEVNVTSMMLMAKYAVPAMRDNEPEAGVRGSIVNLGSVAGLRGGTPSLLYPTSKCAVVNMTRAMAAHHAKDGVRVNCVCPGMLYTPMVYDAEGGMSAEVREARRRRSLLQTEGNAWDCAGPVRFLAGGEARWITGTVLTVDAGATCALAVSMPDGE 4DC0 Chain:B ((21-214)) -----------MATQDSEVALVTGA-----TSGIGLEIARRLGKEGLRVFVCARGEEGLRTTLKEL---REAGVEADGRTCDVRSVPEIEALVAAVVERYGPVDVLVNNAGRPGGGATAELADEL-WLDVVETNLTGVFRVTKQVLKA--GGMLERGT-GRIVNIASTGGKQGVVHAAPYSASKHGVVGFTKALGLELARTGITVNAVCPGWVETPM---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1011 -20151 -19.93 -105.50 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -19.93 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_4DC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dc0-query.scw PDB file : Tito_Scwrl_4DC0.pdb: