Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence CDFIIKQNGTQITSGNIWAGATASFLVS-----GKWAVVSATSE----CKLSLSGLPNNESYTILP---- 1TYG Chain:B ((22-86)) ---MLQLNGKDVKWKK--DTGTIQDLLASYQLENKIVIVERNKEIIGKERYHEVELCDRDVIEIVHFVGG

General information: TITO was launched using: RESULT: Template: 1TYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 135 3015 22.33 57.98 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 22.33 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_1TYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1tyg-query.scw PDB file : Tito_Scwrl_1TYG.pdb: