Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE 1Z9M Chain:A ((38-141)) ------------------QDDSQPWTSDETVVAGGTVVLKCQVKDHEDSS-----LQWSNPAQQTLYFGEKRALR-------DNRIQLVTSTPHELSISISNVA-LADEGEYTCSIF-----TMPVRTAKSLVTVLGIPQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1Z9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 502 -27089 -53.96 -260.47 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.46 3D Compatibility (PKB) : -53.96 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.100

(partial model without unconserved sides chains): PDB file : Tito_1Z9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1z9m-query.scw PDB file : Tito_Scwrl_1Z9M.pdb: