Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIKLGKKISEGKTVDVYESGD----LAVKVFKPD--APKTVVFYEAFMDSKVEETGLNVPKIKEVELIDGKWAIATELIKGKTLAQIMQEEPENCDGYLDDMVELQLSIHAKKVTGIS------KL----KDKLREEIESLD---V---IDHIKRYDLLTRLDS--TPKHVKLCHGNFGPENIVVTDDNKVYIVDWIGASAGNASADVAKTYLKLALTS--TETAEKYLNLFCKKTNTAKTYVQEWLPIMAASTLAKGVTSKEEKDLLFTWLDVVDYS 1L8T Chain:A ((15-246)) --KYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG-KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED-EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI--------------------------------------------

General information: TITO was launched using: RESULT: Template: 1L8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 884 55457 62.73 269.21 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 62.73 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_1L8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1l8t-query.scw PDB file : Tito_Scwrl_1L8T.pdb: