Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVFKNNIALGKSVTEALEIL-ERELG--SQYCYCKTLEGCSNEVHIIKDVRSGLPYI-MRFTRQSRFQNYSLEREI-L-SHVVGRGVATSQ-AYLFAD------GIVA-ACIEGHCVESDKMLG-DSPYYELIA---KQMRRL-HEISVQDDGTSVMYSETHYGLKSMLDISVDYIGKGREAELLYKLYSED-GVL-GQLVNDHPSLL--WTCISHNDLHSGNI-IYSPSAQEVRFIDWEYSTYSINA-FDIACFF-LEFT---------GIDCEISAFPCASKRQDFYLYYFGSSSPP-IDFLCLFF-IPLACLFWAA-WSSGIDGLDVYTKNRTRLGHATLRKLVNE-IW-PQCGLTLDKEDCELLELVDFTFQSLYTN 3DXP Chain:A ((20-359)) -----------RFDTEALEAWMRQHVEGFAGPLSVEQFKGQSNPTFKLV--TPGQTYVMRA-------KPGAIEREYRVMDALAGTDVPVAKMYALCEDESVIGRAFYIMEFVSGRVLWDQSLPGMSPAERTAIYDEMNRVIAAMHTVDYQAIGLGDYGKPGNYFQRQIERWTKQYKLSETES-------IPAMDSLMDWLPQHIPQEDADLTSIVHGDYRLDNLMFHPTEPRVLAVLDWELSTLG-HPMGDFGYHCMSWHIAPGQFRGIAGLDHAALGIPDEASYRKLYEQRTGRPITGDWNFYLAFSMFRIAGILQGIMKRV-------VDGTASSAQALDAGKRARPMAEMGWEYAKKAKQ------------------

General information: TITO was launched using: RESULT: Template: 3DXP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1313 -1697 -1.29 -5.87 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -1.29 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.157

(partial model without unconserved sides chains): PDB file : Tito_3DXP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxp-query.scw PDB file : Tito_Scwrl_3DXP.pdb: