Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSYAHLNHYISPSAEAGLSDAWRRSLGRLGDYSAILIRSLNLRPLEVSLHDQTEEHILVRIRLPG---------SYLILRVAPE------DDLAAYVYQIRTLNGHNIPAPQIIQRDLSRSLVPFAFTLESYVPGVTAIELQSAPLL-RGAGRQAGRALRRMHRIPVTSAGRPTASGRWTQQNWRTVLRQIGRRLAAP-----PADALIFG------EREQRVVADLLDHPVLECQQPSLI-HGRFSPRAVRCTSSGNVHVEALIDPGHYVGGDGLYDLACGLCAA------------------YP--EPWREGLLDGYQSAG--PLNEAERRRLPLLQLLTNYWEACRLYM------HAEPHE----AARSEAIRLMEEQVGLTRLVSV- 1NW1 Chain:A ((32-426)) -GMKELLSTMDLDTDANTIPELKERAHMLCARFLGGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTLLSERHL---GPKLYGIF-SGGRL------EEYIPSRP---LSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEP----DYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHIS----LSKSPVTFCHNDLQEGNILLPKRLV-----LIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRLSLYFKHKQLLKNLA

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1275 -42531 -33.36 -139.90 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -33.36 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: