Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSIPSPSSCPARVDVPDALAALARFITLRLGCERVQIDACQ--RLSGGAIQENWLIRARVLNGGLQADRRWVLRTDSPSSV---AASMERSQEFAVLRAVHQ-AGVKVPEPLWLCQDATVLGRDFFLMQALGGSANGHRLST-------DPELEPMRKGLCHELGASLARLHGITPDHPELGFLPTPAPDPVQASIDQYRHYLDALP---GSHPVIEWGLRWCELNRPQ---PRPSRLIHRDYRTGNYMVEAGHLAGVLDWEFAGWGDPREDLGWFTARCWRFARPDRE--AGGVG---ALEDFLGGYQEESGYRPDRDELRYWQLMAHLRWAVVALQQVQRHLSGQQQSLELALTGRMVPELEQEILKLSGAPT 3ATT Chain:A ((3-326)) -------------------PAVISRWLSSVLPGGAAPEVTVESGVDSTGMSSETIILTARWQQDGRSIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGELTDVPVPRVRWIETTGDVLGTPFFLMDYVEGVVPPDVMPYTFGDNWFADAPAERQRQLQDATVAALATLHSIPNAQNTFSFLT---DTTLHRHFNWVRSWYDFAVEGIGRSPLLERTFEWLQSHWPDDAAAREPVLLWGDARVGNVLYRDFQPVAVLDWEMVALGPRELDVAWMIFAHRVFQELAGLATLPGLPEVMREDDVRATYQALTGV--ELGDLHWFYVYSGVMWACVFMRTGAR---------------------------------

General information: TITO was launched using: RESULT: Template: 3ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1337 -26768 -20.02 -90.74 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -20.02 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_3ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3att-query.scw PDB file : Tito_Scwrl_3ATT.pdb: