Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGNIKRTKQLKSIIRQHNKS--IKYVKQINRGYYGYVFLITDPFGKKSVAKVYKKD---DYAKREMAQLEMLAKH--SLAHIPEIYAIDTKKQNGFFDILFMEFIDGVDASR--IKVIDEKEKMRLSNEIVDNLIAIHSVSNPNGFGDFVSGNYFERWQDYYKLQIDSLFGSLKSDRPKKFSKQSFSIAEKLYYSFDDVFCLEVKQNSLIHGDYNLWNLMVDPATNRLAAVIDPFGCSFADRELDLFQLQNSN------GNEYRLLENYASKIPLSEIFEIKNAYYRFWDDIKHLVNVGYCDNDSFCKYGNMTLELLNR 5IQI Chain:C ((9-282)) ----ATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNN----EYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISD---ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTD-ISECTI----DNKQNVLEEYILLRETIY-----NDLTDIEKDYIESFMERLNATT-VFEGKKCLCHNDFSCNHLLLDG-NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGN--IDIEKAKEYQDIVEEYYPIETIVYG---------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1114 4532 4.07 17.50 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : 4.07 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: