Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQAQFEKVIEVLFPDIIIQEVTAYEKGWDNDIFIVNKEIVFRFPRSEEVASKVKDEIILLKHLAHKEP--KLDIPRYKGIEMDGNLKCVKYNFMKGKSLSEIKNS---R-LDLHSAELLGDFLSRLHSIELSALKETNITSFHTDTYWENLYESAKTYVFPNITHSERAEIQQFYEDFSNNPIYSNVNKAVIHGDLTAANILYNKEEECVSGIIDFTDAQIADPAFDFAGLYWA----YGPEFTKKLLTYYDGKDKTGIFERVQSFYGLQPIFHELIYAVKENHDVDWGSALERFQYLNSHR 5IQI Chain:C ((11-302)) -NVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGFAKEKAIYNFLNTN-LETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTI--DNKQNVLEEYILL-RETIYNDLTDIEKDYIESFMER-LNATTVFEGKKCLCHNDFSCNHLLLDG-NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQ-DIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRT---

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1253 -13777 -11.00 -48.85 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -11.00 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: