Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDISIIAAQLVKEKVIS-HNPNSVKALNGGTTSTVYLL---DEKYVVKLNEAE-----VIREEAHFLSFYEGNTLF-SKLLYKEPLHTYIVYSFLEGS-TWCEQGHKRSTLRTLVKEVINKYKIVPEADGWGWKENPVQSWNEFLTTNVMEAYENVRRYISEEEYRTVLKLANR-DAGVNQPFLLHGDCGFHNFIFRENRLHGVIDPL-PVLGEPLYDLIYAFCST--PEDLTKETIAYAMKQCLFHKKERDLYEEIVIGLYLRIDTCLRHHPKDLEEYLVAWRYWMDEINPAL 4R78 Chain:A ((23-252)) ----KIIKEKISSLLSQEEEVLSVEQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDI-EAGIKVNEYIESAITLDSTS--IKTKFDKIAPILQTIHTSAKELR-GEF-APFEEIKKY-ESLIEEQ---IPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQ--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 882 14598 16.55 67.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 16.55 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: