Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIINEEVIWIVNSLVGEFEIIKDHRNSSNRTGVLEINANNKRMFIKIHNR-----LSRWSPEVYAYKNWTHILGEYAPRLIHSFNDDNFYGIITTPIYGKTVNEYQINDEDILEPIYYKAGELLKQLHNNFKGTYFGIPAIDGSPLESKAKTDPVDYINSALEDILKSGYDKGLFNNSDKELVKWCMKHSDV-FANSKPVPTNWDFSQNNWMVDEDGKFTGFIDFENMLWGIDVDSFGIVIERYTP--NRPKLRKALFEGYGLENSEEKQLQLKIVSVKMAIADITYGASVGNDRIFSLGRNLMDNLKMPGFKIH 1L8T Chain:A ((9-246)) ----------LKKLIEKYRCVKDTEGMS-PAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG--KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDE-QSPEKIIELYAECIRLFHSIDISD-CPYTN---------SLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKD-GKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1L8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1005 38749 38.56 168.47 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 38.56 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_1L8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1l8t-query.scw PDB file : Tito_Scwrl_1L8T.pdb: