Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAPFSIKQADIQQQLTAAIQKHFPAVEIADIRFELVGGLSSKSWRIRGPGIEWLARRQSPTERKMGVDRQREFALLRRMSAIGL-APRPLLWRD-DWLIVEWVPGR-IATSDEFLMMLANGEVARVLSQLHRLPRSGHP-LDIKMLFAQHWQLMDPHRR---SPVLLRAHHYFQRAPLPTPLALAPLHLDVHAENLLITP-QGTMLIDWEYASDGDIAFELAFIVRASQMDSMAQRYFLQSYQRHRRGFSVNRLQQQMRQWFPWVDYLVLMWFEIRWQQTKNPEFLADIPALYHRLQLGHRLQPD 4R7B Chain:A ((19-295)) --------SHMEKIIKEKISSLLSQE-EEVLSVEQLGGMTNQNYLAKTTNKQYIVKFFGKGTE-KLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDST--SIKTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQT----PVSHEKIAIYKILQDTIWSLWTVYKEEQG--EDFGDYGVNRYQR----------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1238 -36628 -29.59 -136.16 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -29.59 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: