Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVMRSPLLLAALADAAVPGIRPIKVEGLPVPAGDLYQSAHITGA-DGRVWLVRSALTGAAGAELAASDALVQLLTRRVDFDVPRVEGSARPKEGSAVAVYARPRGRALDWRHLA---------GRSAAARALGQALAHLHGVDPRVVEEAGLATYDADTYRQRLRADVDRAAATSRVPSALIARWREALDTPALWRFSTTVTHGPLRGADVHVDEGEVSAISGWEHAGVADPARDFALLWNSAPQQAFDTVFEAYAAERAERPDPNLERRVRLMGEMEL--AYALLASRTMGDDARVERHAAALEELAFEVEGDSSLLPPARRAGTAPIDLVPPLDPADIEVVDAEVGEEDATVEIPVRLAPEREDRSEHAGATRVDGDAARTRLPHGSSEEPAASSDDSAGAVADESSGGNARSR 5UXD Chain:A ((1-297)) QSMPEDLDALLDLAARHGLDLDGGTLRTE--EIGLDF-RVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIST-----SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVRSPAQVRGAWRQDLARVGAEFEIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSAPSAIFEVALQAYAEGGGR-PWPGLARH--CTEMFSAAPLGYGLYALATGEAAHREAAAAALNPP-------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1279 -7174 -5.61 -25.17 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -5.61 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: