Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence LKPRFTLAEAKAMARDEFHV--RVAAIVELN-SYDDQNYKVTAENGAVYVLKVHNGVESR-DEGLLDAQSQLYERLRRKGVAAPYPVDSNP--LADGDATFALRLLEWVDGDLLSSKEITPGLLATTGAFLGRIRLALDDFDHAALHRVHLWDGR-QFPALASFADGLVDAG--CDVDKAA---DVHRAIANFDAVVAP-AAAQLPLATLHGDFNDANVLVGA---ETLGVLDIGDSVHSWRVNDVAVGMAYVVV 4WH3 Chain:A ((5-248)) ------NEVLFGI-ASHFALEGAVTGIEPYGDGHINTTYLVTTD-GPRYILQQMNTSIFPDTVNLMRNVELVTSTLKAQGKETLDIVPTTSGATWAEIDGGAWRVYKFIEHTVSYNLVPNPDVFREAGSAFGDFQNFLSEFDASQLTE-TIAHFHDTPHRFEDFKAALAADKLGRAAACQPEIDFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFG--

General information: TITO was launched using: RESULT: Template: 4WH3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 869 13783 15.86 60.45 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 15.86 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.317

(partial model without unconserved sides chains): PDB file : Tito_4WH3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4wh3-query.scw PDB file : Tito_Scwrl_4WH3.pdb: