Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSFILVFLYALIYRNGGCRLLDKWLKAELEKKIGA--IQHMSLLDEQGGTSTVRKIVTSQ-GAFLLKSSSRQRYREWLRREALVLQKLTETHRLPVPVYYGFIQQQHSSHIVMSFEDGITLTSALRKAK----GDTEKKKLIKSFGQFLNRLHETELVPDLQP--EIDWLDLQIKRAGEYVEKG-----Q-AEGSAGLLKELDQHRPVPVQQTMIHGDCTTDNVLVKDGEVCLFIDAAGISAGDPRYDESLAIRNFATNEAFLNAFYEGYCRYRVSKQEFEYFNGGLYEFF 5UXD Chain:A ((10-252)) -------------------------LLDLAARHGLDLDGGTLRTE-EIGLDFRVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIST----SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVRSPAQVRGAWRQDLARVGAEFEIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSA--PSAIFEVALQAYAEGG-----------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 914 18654 20.41 81.81 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 20.41 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: