Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKLYEHDITAIAWQDKHELLERILQQAG----TIAITPLMQGMEAEAVKVATDADRYVLKIWSRDS---KPDI-RLQYQVMNMLHERGISVSKPIGWGI------NASGHSVLLTTFDGDPLRDG--NAHTMREIANLLDHIHRISPEEIDRH--TR-------LPRY-NFRDYFFPGMH----EYPDLYPL----LEQL---VELA--SPTHKRIIHGDFHLGNIVEQQ--GRLT-VIDWTNVQLGDPRYDFAWSLTLQQFY------------IPEHFAEVFRDTYVA--MNEIQREELEVFQALACLRWMLLYRNGGVPMGADTLERVRRLLTSNPHLHGVTI 4OCV Chain:A ((24-316)) -----------------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSG--------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 948 12706 13.40 53.61 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 13.40 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: