Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAVEHILGEDWTVVPA-GGSTGTAYYAKSD--QRRLFLKRNSSP------------FLAVLSAQGIVPKLVWTKRLENGDVLTAQQWLDGKALNPEEM----QHPRVAKLLSKIHHSSELLDMLLRI-------EKQTQMPTQILAKMKEKVKTT--DDA-----------DCSLVIHKALKYLEDRLPYVYYSKPVVCHCDLVHHNWMLSK--NGELYLVDWDNAKVGDPAMDIGRILQSYI----------PKQEWDQWLSHYGEENN----AHFMQRMHWYLMIDAIHGYTWNVRRDKMKVAHQHMSELQQLLDQVNDWNL 2QG7 Chain:E ((110-432)) ----------SLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIAKKIYVFF--TNG---RIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCK-RENSPIVLCHCDLLSSNIINTVGG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMH----------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 885 12736 14.39 55.37 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain E : 0.67 3D Compatibility (PKB) : 14.39 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: