Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------QKCIITIRKADPALGQDTLYDLVSLAPNSDLTFGKIPASMTEVTFGVDEKCQARLVRGDLSRPYEYKGMQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 5F7C Chain:A ((12-723)) LFDFDTELLRDESLWKACKPTAVYEKDGDICVTVPFQKQLLANDMVADTAVPREEYTLIIRQYNIGITRLFLGFGEYEILFTQDGTKRAVINVE--EPALDRWS-----ELLPD--------PQETLDITLYPDGKREIRLAAYDHFSPPRYDGLPIAFCKRTGKKERATLSFESRPDECFAGTGERFFKMDLSGQTLFLKNQDGQGVNNRRTYKNIPFYLSSRMYGTFYHTCAHSKLSLAGHSTRSVQFLSDQAMLDAFVIAGDTMEEILRGYRDLTGYPSMPPLWSFGVWMSRMTYFSADEVNEICDRMRAEHYPCDVIHLDTGWFRTDWLCEWKFNEERFPAGTIDFTYPKATEWYKGLLKQLLDMGVTCIKTDFGENIHMDAVYKGMKPELLNNLYALLYQKAAYEITKEVTGDGIVWARAAWAGCQRYPLHWGGDSCSSWDGMAGSLKGGLHFGLSGFAFWSHDVPGFHTLPNFMNSIVAEDVYMRWTQFGVFTSHIRYHGTNKREPWHYPAIAPLVKKWWKLRYSLIPYIIEQSKLAVESGWPLLQALILHHPEDKLCWHIDDEYYFGNDFLVAPVMNSENRRDIYLPEGQWVNFFTGERLQGGRWLKEVYVPLEEMPVYVRENAVIPIYPEEV

General information: TITO was launched using: RESULT: Template: 5F7C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 128 9257 72.32 168.30 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.50 3D Compatibility (PKB) : 72.32 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.336

(partial model without unconserved sides chains): PDB file : Tito_5F7C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5f7c-query.scw PDB file : Tito_Scwrl_5F7C.pdb: