Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFL-IEHQ-GQRFVVRQPHDPDAPQSAFLRQYRALSQLP-ACI-APKPHLYL-------RDWMVVDYLPGE----VKTYLPDTNELAGLLYYLHQQPRFGWR-IT---LLPL----LELYWQQSDPARRTVGWLRMLKRLRKARE-PRPLRLSPLHMDVHAGNLVHSVS-----GLKLIDWEYAGDGDIALELAAVWVENT-----EQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ 3CSV Chain:A ((4-301)) ---SREDEIR--DFLATHGYADWNRTP--------RYQRLRSPTGAKAVLMDWSPEE--GGDTQPFVDLAQYLRNLDISAPEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFE---

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1124 -3341 -2.97 -13.05 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -2.97 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: