Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTTNPAAPAAPLAPPDPARIAKTVATKMPFRGDMTGLTPLAGDASNRRYFRIALKGTPSALILMQLADPEGFKKSEEAVSGASAQIAELPFTNVLAHLQR-TGVTVPQLHYYDR------------EAGLLYLEDFGDLTLAEACRDADHARLVALYRQAIDQLVLLQVKGTKPPAPGCIAFHRRFDVPLLMWEFDHFLEYGIVARLGQPMKPADDKAVRAEFQRIAELL--AGQSQVFVHRDYHSRNLMVDG----SRIGIIDFQDALMGPATYDLASLLKDAYI-ELDDAVVDGLVDQFLDGLAAHGQGWADRAAFRRLFDFTSIQRNLKAAGRFVYIDRVKHNPKFLADIPRVLGYVKRNLSKYPELATLRQHLAPYVAELQ 6EF6 Chain:A ((27-321)) -----------------DVTIAQQALTHYDVS-DNASLRLL-NLSENA-TYLVEDGE--HQSILRVHRQDY--H----------QPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESL------TLDDFQTLGRITASLHEHSQRWTRPAGFG-RFSWDWEHCLGDTPRWGRWQDAEG-VGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRR------ELPAADE-AMLPSFVFLRRLLLLAWMGSH---------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1244 -49522 -39.81 -182.06 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -39.81 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.213

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: