Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAFDVLARLGVD--PPDLELLRFG-ENALYAFRER-R-LVVRVARPSTD--PTHV-AQTVEFVRRVAARGMPVSEPADLTELVQPVV-TSTGVVTLWTLHDVDEG-----CRVSAAELGGLVRQFHELAAPEADLL-----DEWE-PFGLIRSRLAAAERD---GFD--RSLTGPLVDLLASLETAVDRLS-ARLGIGVMHGDMHYGNVLCLP--GRRLLLIDFDQVCRGPREWDLVPNLVTVRRF-----------GLAESDYQEFCAAYGF------DLRTSPDVETLVRLRELGMVSWLLQQYGTSAVIDDEIRLRVGTIGESVGPPATRWWAH 4OCV Chain:A ((27-326)) -ALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRT-TSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMR------------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1048 -10087 -9.62 -39.55 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -9.62 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: