Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNRVSSALEDAFPNRTVD--NVS--SAGISWNAQNRTVAVDFGD-------GQRVFLKVAVNGD------RSRIERERAVISYVGANCAVSVPNVIATETTG---PVPYLATEPVSGQPLTTLLA---------DATRTERAALAHDVGSALARVHDCRFEEHGHVVGGDADHLELDTGTWTDVLVETISEMREMAP-CDRFDQHFEMVSDAVERNRDRLD-RASATLLHGDPACPNCFRTGD-GVGFLDWELSHVGDPVRDRSRTLDQQFDSLREGS-----PE---GVVSAFYDGYRSQAGGLPSGFEERRPIYEVIRLLSVSGYFERTADYRDESRSDLAAWLEAEMRRRLDAIRR 3ATT Chain:A ((4-317)) --AVISRWLSSVLPGGAAPEVTVESGVD--STGMSSETIILTARWQQDGRSIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGELTDVPVPRVRWIETTGDVLGTPFFLMDYVEGVVPPDVMPYTFGDNWFADAPAERQRQLQDATVAALATLHSIPNAQNTFSFLT--------DTTLHRHFNW-VRSWYDFAVEGIGRSPLLERTFEWLQSHWPDDAAAREPVLLWGDARVGNVLYRDFQPVAVLDWEMVALGPRELDVAWMIFAHRVFQELAGLATLPGLPEVMREDDVRATYQALTG-VEL---GDLHWFYVYSGVMWA----------------------------------

General information: TITO was launched using: RESULT: Template: 3ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1140 26123 22.91 97.11 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 22.91 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.261

(partial model without unconserved sides chains): PDB file : Tito_3ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3att-query.scw PDB file : Tito_Scwrl_3ATT.pdb: