Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYPGINETQLELIFSRYG-K-DCKIEPLQEEASTRRYFRITSVNGKEEVVCADET-----VNEDFIVISEFLNAN-GIRVPRIHDTNQN-------LGLTFMSFEGLKDFSTYNLN-DYKNKFPILIDLILKLQSLD-PPSLVK-TRKFDTEKLSFETNLTLDKFEAFRKQFQIKTDITNEAKAFIEETVGY-LNKYPINVFTHRDFHCRNILIAP-NTDYALIDFQDARMGVPQYDLASILYDAYYPLPRDFRSLMLKSFRERNVDQTKKFNDTFYLQALQRSFKALGTYFRMVTDHKKDKFKTSIISCLNQLEEIIQLGMFADSLYIFVRSLREELSRHKEFKNL 5IGI Chain:A ((4-261)) -VTTADTSQLYALAARHGLKLHGPLTVNELGLDY-RIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRRE---FV--VNDKR--LHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF----GEEGLAKLLLTYEAAGGRVWPR----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 983 10566 10.75 44.58 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 10.75 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: