Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDATLARSLPALLADLWGLEDVDVRPLGGGMNSATCLVTATGGDRFVAKWS-SDAESLEAGCSAATLLGRQGVRTGEPVGSTAGAPVVAMAGGALALLRHVPGRELDGDSGHEQQLIGTALATAHTAGCPASDTG--P---FMSEWLY-PAEDVLAVA-------------------TWLPPALA---EIRREYN--------ALPQLTWTQLHTDPAPEAFIHDDATGSTGLIDWAGSRRGPALYDVASAVMYLG------GPQQAEQFLSSYADRGPVENDELALLDIFRRFRWAIQASYFAGRIVADDRTGIVDDTDHNTEALARARRGLAEAR 2Q83 Chain:A ((19-305)) ------LTELAENVLQGWDVQAEKIDVIQ----ALVWKVHTDS-GAVCLKRIHRPEKKALFSIFAQDYLAKKGMNVPGILPNKKGSLYSKHGSFLFVVYDWIEGRPFELTVKQDLEFIMKGLADFHTASVGYQPPNGVPIFTKLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLG-ENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRAPLTEEQKQVMFIDMLF-------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 957 -32004 -33.44 -133.35 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -33.44 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: