Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence RDCRLYYCWGPGSDNRRVVGTVDKGGSWK-----DYVNGETITVKAGKDCKAEIIGGKTCDMMPSFSWCLVNA- 1WAP Chain:A ((8-75)) ----DFVVIKAVEDGVNVIGLT-RGTDTKFHHSEKLDKGEVIIAQFTEHTSAIKVRGEAL-IQTAYGEMKSEKK

General information: TITO was launched using: RESULT: Template: 1WAP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 238 -6347 -26.67 -102.37 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -26.67 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_1WAP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1wap-query.scw PDB file : Tito_Scwrl_1WAP.pdb: