Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PEHCFVRIL--KYEIPIHGDGYLVGSDVNWWVGNQKVDFHINND-------CTIQRRGGTQEKK------ 1SRZ Chain:A ((92-159)) -EAEFVRICSKSYLTLENGKVFLTGGDLP-ALDGARVEFRCDPDFHLVGSSRSVCSQGQWSTPKPHCQVN

General information: TITO was launched using: RESULT: Template: 1SRZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 157 2669 17.00 50.36 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 17.00 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.056

(partial model without unconserved sides chains): PDB file : Tito_1SRZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1srz-query.scw PDB file : Tito_Scwrl_1SRZ.pdb: