Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKSKELEIVKK-LTNVADENNIVLNEDGWTSRVYIVNKGEIVLKFLKNKQYREEFEHEINILKLIKDHKFNVNIPLISSSEEDNTYIVLNGLMGKSMSAEIVDALTKEEKRKIGTQIGLFLKKLHTVAYQGKSPNNE---SEIFEWFQKSFNRRKRTLK--KHFNENELVAIEDLVTTLPQRSQKLGIVQVFCHGDLGYNNILLTDSLEVGVIDFGDAGYNDQSYDFVGL-------EDDDMLDAVILAYDG--DEILKAKVEIRRQLLPLMEMLFLIDKKDKEG-VQQCVKRVRINLKMHEIYNG 4DCA Chain:A ((28-315)) -------EIYEHLNKQIK-INELRYLSSGDDSDTFLCN-EQYVVKVPKRDSVRISQKRELELYRFLENCKLSYQIPAVVYQ--SDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILSNAVLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKDVSELLQTQA--------

General information: TITO was launched using: RESULT: Template: 4DCA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1125 28681 25.49 105.44 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 25.49 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_4DCA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dca-query.scw PDB file : Tito_Scwrl_4DCA.pdb: