Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADTPPLTLPEALRRTLPAARWELTHTNDAGTRVYR------SQRFLIKVHPRGP-SHPLFSEKERLRWLAPRVPVPPLAGYAEDADHAYLALARLPGMPM-----NHPDARLHARRNADLLARALAELHALPIRDCPFTHTLTERLRDLRTR----LTASGEIPT-----PVAERFNALVRQRPHDEDLVVTHGHATLEHVLVNGEYVEALTGVGRAGLADRHVDLAAAHTSLTQDYDPDAAAHFLDTYGRARIDLHKLDYYARLNALA 4FEU Chain:A ((20-271)) ------------LDADLYGYRWARDNVGQSGATIYRLYGKPNAPELFLK-HGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDS---GENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGE-FSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEF-

General information: TITO was launched using: RESULT: Template: 4FEU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 977 30143 30.85 130.49 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 30.85 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4FEU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4feu-query.scw PDB file : Tito_Scwrl_4FEU.pdb: