Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQQVISFLNENYPLHIHTAK---AVTNEMYRCIDDQGTYYARITNYKPYEEQLEEVSWTNALYREGVG-VAPAIPSCRGQIVELMPPKDIIVVVYKAAPGIHLPRG---EWNADVLAELGRQIGRMHRITQSYEANHRLHH----------LGDWHDQEEYN---------FEQYIPAEETAIRAIAANVLAEVKKLPRERATYGLIHGDLWLENILVDRGSSLTMIDFQDCEKHYYMYDLAVPLYSALEFSFAGAGNIRDYGISIANALLEGYQEEHTLDPEMLKQLPLFLKLKEIFEYNLMNMYWDRERLSEEQIRIMNLYRLRIEHHVQLLEWD 3I0Q Chain:A ((9-258)) --QQLIELLKVHYGIDIHTAQFIQ----AFAYQADSESKSYFIKLKYGYHDEI--NLSIIRLLHDSGIKEIIFPIHTLEAKLFQQLKHFKII-----AYPFIHAPNGFTQNLTGKQWKQLGKVLRQIHETSVPISIQQQLRKEIYSPKWREIVRSFYNQIEFDNSDDKLTAAFKSFFNQNSAAIHRLVDTSEKLSKKIQPDLDKYVLCHSDIHAGNVLVGNEESIYIIDWDEP--------MLAPKERDLMFIGGGVGNV------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3I0Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 660 -7755 -11.75 -36.41 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -11.75 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_3I0Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3i0q-query.scw PDB file : Tito_Scwrl_3I0Q.pdb: