Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRDAHERLTERFEDYRIVRKLRRVPPHEVYEASVDSRRAVYKGDTGPTGNAAVEGRVTALVGRETAVPVPETLRVGDDCYVTAWHPDAPAPDSSDEADEAWAAAAGRGLATLHTETEPLVDGCGPFRAADGGVRVETADDWHAAAVDHVRHRRSVLAEYGHADVADAAVEFLRDRPDAFDGAGDPVCCHGWWTPEHVAVEDGRVACVVDFEHATAAPGEWDYWRTVTPTFAACDEDTTAARRAFRRGYESVRPFSPGFERRRPYYGLLNGVYYLQSLYVQDRHGPAATAERAARFRARIFETISELDRGRLGRTAHPSRRVAFARHTPTSVMSRFDAVLFDLDGTLCENEQDVEHLYFGAFERAEV--------EPFGAPSDLWAVLEGPPTTPDEFEYYREGFRTVAAEHGRAEADVSAVAREFVDGIDYGAVSLLGGAEAALADAGVGHRVGLMTNGPEYRQSVKLEALGLEDAFEVVVYAGDMERRKPHTDPFDRAVEAL-GVDASATLYVGNSLKYDVAGAREAGLPVAWY-PDGDADPGSYDPDYVVESLAELADVLDGTVE 3I76 Chain:A ((4-230)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KRYRTLLFDVDDTILDFQAAEALALRLLFEDQNIPLTNDMKAQYKTINQGLWRAFEEGKMTRDEVVNTR--FSALLKEYG-YEADGALLEQKYRRFLEEGH-QLIDGAFDLISNLQQQFDLYIVTNGVSHTQYKRLRDSGLFPFFKDIFVSEDTGFQKPMKEYFNYVFERIPQFSAEHTLIIGDSLTADIKGGQLAGLDTCWMNPDMK-----IIPTYEIRKLEELYHILN----

General information: TITO was launched using: RESULT: Template: 3I76.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1032 7570 7.34 35.71 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 7.34 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.172

(partial model without unconserved sides chains): PDB file : Tito_3I76.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3i76-query.scw PDB file : Tito_Scwrl_3I76.pdb: