Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGDYSKLHPKTAQRILNQYGIEDEFTLKALSLGISNSNYKVDTGNKQYLLKVSNDKGFA--ELCDEQHILKHLK---DLGFPYSICALPTIKGELVYKQEDLFG-----VLFPFIKGIPPGPSDITCREIGRALGKLHATTKDKELDVREYAAIGQDAKTIREFIDDPSCPADFSEA-------------FHQVFSDDLKGYLSVNKTRCLIHGDLYYDNTLFWDESIA----VLLDFEQAGLGNALLDLGISISGSCIEKGQINPQLVESFCEGYKLSHALNDNEKNYLADAILLGLFSIALWRIKRFKHGNLNPMLENSYRDLLNKAKSFKTIIDSKGLKFEL 6EF6 Chain:A ((31-317)) -----------AQQALTHYDVSDNASLRLLNLS-ENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVP---TVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEESLTLDDF-QTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLV-DSSTPQRTITVIDFDDCGFGWYFYDFGTAV--SFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAW-----------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1138 16826 14.79 65.47 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 14.79 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.308

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: