Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence RICKVGIYRAAKDNNWRLRAARKIRISMDNTPMQFQW---DYKDGASKEATYKVELHYEGCEAKVVGDQKLPAYLTIKGEI 2J8G Chain:A ((56-101)) ----IGFYHFARFGGDVAEAEREAQFFLDNVPMQVKYLVLDYQDDPSGDA-------------------------------

General information: TITO was launched using: RESULT: Template: 2J8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 92 4309 46.83 100.20 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 46.83 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.075

(partial model without unconserved sides chains): PDB file : Tito_2J8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2j8g-query.scw PDB file : Tito_Scwrl_2J8G.pdb: