Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSEALEQFVVEEAGVGAPRGASWSRVDEGQGANRVWRVRWGPGSSVIVKRCGPGRAFVQERRAATRWAPAVADASRGLGHARVLAASQALRCLVFVDA-GRGEEQVA-----------LSPAAFACAGRYLARLHALPVDERDPVP----LVEALARREARWLEGAPE---LRA-VASSLGPGALAD-A-DWGPRVPCHRDFQPDNWLWD-GRTLTVVDFEHARPDARALDLAKLWTRLDGDVDARANAAFWSAYLGPEPCSPS-FLAQLRAGLVLHGLASVAWGRSHDDEAFVAEGERAVALARSGREPPNPRAGTPLASL 5UXD Chain:A ((8-298)) ---DALLDLAARHGLDLD--GGTLRTEE-IGLDFRVAFARAHDGGDWVLRLPRRPDV-LERAAVEGRLLAMLAPH-LDVAVPDWRIST--SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEVRSPAQVRGAWRQDLARVGAEFEIAPALRERW-EAWLADDGCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSA----PSAIFEVALQAYAEGGGRPWPGLARHCTEMFSAAPLGYGLYALATGEAAHREAAAAALNPPE-----------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1205 -53377 -44.30 -199.91 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -44.30 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: