Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRRAELDDDLGRRRAATPAPQRVEELLAVELPAVGEELDGAVVPIDYGTSTELTVTAPDLFAALSRHPRAGRIVASVGDVRRLSGGFSKEMLMARATYSDGSGERFVVRKVAPGRR-ADGLRAEYDVLSSAFHSGCPVPEPWWYDSEALGTPAIATSALSGT-PAGDPNGWVAPPSSAVLASVARAAAQQHQVELAAFDANPLPPLISAEDRREALSERRAVLDGLWAEGDD-AWAPAFNLVLNWLESAIPEPADPPVLVHGDFGPHNFLIEGDELSGILDWERSHAGLAVEDLAYLRPTLD-D-ESWTAFMREYVAAGGREPSDDALVWYIVWQDFWRAVSAYRMRSLFLQSPDQVLYGISGLLLAPRFLTSAVRSVSSAHAVAGARAC 4R78 Chain:A ((18-280)) -----------------------------------------------------GSHMEKIIKEKISSL-LSQ--EEEVLSVEQL-GGMTNQNYLAKTT-----NKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDI----EAGIKVNEYIESAITLDSTSI------KTKFDKIAPILQTIHTSAKELRGEFA---------PF-EE---IKKYESLIEEQIPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQTPV-SHEKIAIYKILQDTIWSLWTVYKEE------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1200 21244 17.70 82.34 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 17.70 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: