Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHDIAALLHKAAPRSEIAEVLPRSGGQLATVFEVRRIA-AAPLIIKLYSPEWAWKQAKEIHVYDLLAPHLGTA-IPQVVHAEPEG-----ETHAFTVMTMLTGVPLCETTAPDYQVVYKQLGELLTAIHRIPQPAYGYLT--D-EVLEPLPSNDAYMRRQFAK-----KLQEFEELGGD--RALHARLTEYVEQHSGLF-ASSSRPVLCHNDFHEGNVLVDPDTWQVQGIVDVENAIAADPLIDLAKTQYYSIKN--DQAKLSGLLTGYGELPSDWVERTAVYRLYHALELWDWFRLIGVIDPLESIAADLVSLLGTSRVAGRPDAVR 3I1A Chain:B ((9-292)) --QQLIELLKVHY-GIDIHTAQFIQGGADTNAFAYQADSESKSYFIKLKYGYH---DEINLSIIRLLHD-SGIKEIIFPIHTLEAKLFQQLKHFKIIAYPFIHAPNGFTQN--LTGKQWKQLGKVLRQIHETSVPISIQQQLRKEIYSPKWREIVRSFYNQIEFDNSDDKLTAAFKSFFNQNSAAIHRLVDTSEKLSKKIQPDLDKYVLCHSDIHAGNVLVGNE--ESIYIIDWDEPMLAPKERDLMFIGGGVGNVWNKPHEIQYFYEGYGEINVD-KTILSYYRHERIVEDIAVY---------------------------------

General information: TITO was launched using: RESULT: Template: 3I1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1034 -35780 -34.60 -135.53 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -34.60 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_3I1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3i1a-query.scw PDB file : Tito_Scwrl_3I1A.pdb: