Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKSPDLPVLPGSDQVAPASAIWSSETWRWEAVRWMDTALARRGLERVPTTPLQPRVRPWSTVLVAETTDQ-GRVWFKATSP-----EMSPEAAILRALRSVTPDAVPVVWADDPE-------RGWLLMPD-QGPVLRDVQRDDDAVSLMSSVVRRYARLQRSSTRVVDQLR-DAGVPDMSPR----ESARRWQRL----GLKPDTTREVRRAAQRLDAVGLPLTIQHDDLHAGNVFADGTSAGAHDARIFDWGDASVGNPLCSLLVPLERIGEGLDDDAARAARERILRAYLSVWSDVVSSTALSAAVDDALLIARVGRVFTWQRALTRASADERHAWGVHGRRLIAEINRSLGHAPEPSHD 5IGH Chain:A ((12-239)) -----------------------------------LYALAARHGLKLHGP-LTVNELGLDYRIVIAT-VDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWV----VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNT---ERVSGMIDWSEARVDDPAIDMAAHLMVF-------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 678 3654 5.39 17.82 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 5.39 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: