Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAFDVLARLGVDPPDLELLRFGENALYAFRE-R-R-LVVRVARPSTDPTHVAQTVEFVRRVAARGMP-VSEPADLTELVQPVV-TSTGVVTLWTLHDVDEGC---RVSAAELGGLVRQFHELAAPEADLL-DEW-EPFGLIRSRLAAAE-------R----DGFDR---SLTGPLVDLLASLETAVDR------L-SARLGIGVMHGDMHYGNVLCLPGRRLLLIDFDQVCRGPREWDLVPNLVTVRR-FGLAESDYQEFCAAYGF----DLRTSPDVETLVRLRELGMVSWLLQQYGTSAVIDDEIRLRVGTIGESVGPPATRWWAH 4PDY Chain:A ((25-303)) ---QAVVSKYDLAIQQR---HADGN-IEVWTDSKGRRYAAKRSSIA--PAHCRIMVQCLRHAQEQGFTKFARFVTT-SSNAPYVRHGDFTYYVTEWVSGQPANFGLPEHVAQTAYTLAQFHEATRSFRTDWK---DDVFGLFQARWRDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERGNWDGHLAYACFLHFDAVRNIPKSEYRAVEAILRFPY------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 939 -4108 -4.37 -17.26 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -4.37 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: