Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPHYWPDMPFDYLEWLVPVLPESWLISLYEQAKAQK--TTHLHDDPYVRPVIRISD---KIAVKCGHGV------APGEAATQQYAYQHLDRRVVRVPRVYRYFQVKSN---S---SWPIGYIFMEYIPGKTLEEVDLNNDDISKRLADIVSELATVSGGAIPGQINGGTL--EGYLWGDDGTKDVFRS----VDDMNHWLNRRLKLLNKEI---DLRPYPLVLCHLDLCRRNIKLLDD---DSLCLLDWGHSGFFPRFYEVAAAQCINDD----GAYIRSLSNAVKNKANLSEDEEKCVWLILRARAASLRYIFEPRENNLRTLADIESLPPLPPLPPVEPLPHK 6EF6 Chain:A ((27-323)) ---------------------DVTIAQQALTHYDVSDNASLRLLNLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTD---SDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEESL-TLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGSHTH--------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1170 19764 16.89 75.72 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 16.89 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: