Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDGSASSSSPPSLSQPINDTTPRRLLTLCSVGVARPFQRKRWSPSLLRLS-----PWTCVKLGTF----RDLCEARTIQFIASNTSIPVPKVYCSFQRRDKRDGTTTTYIVMQRIRGQTLASAWPTLSASSRNKILLQLRHFVQVMRNLPWPKREYKISNVDGGSLWDCRLVSGVDRFGPFKDTLAFHLFLRNGL---EK-APPELPEVDEMIKLQARDWGPVVFTHGDLSSLNILVRGDRIVGIVDWETAGWFPPYWEYSTACQVNPRNTFWAEWIDHFLEPWPDALEMERVRQRWWGVI 4GKI Chain:C ((35-265)) ----------------------------------------GQSGATIYRLYGKPNAPELFLKHGKGSVANDVTDEMVRLNWLTA--FMPLPTIKHFIRTPD------DAWLLTTAIPGKTAFQVLEEYP-DSGENIVDALAVFLRRLHSIPVCN--CPFNSDR----VFRLA----------QAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLG--EFSPSLQKRLFQKYGIDN-PDMNKLQFH---

General information: TITO was launched using: RESULT: Template: 4GKI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 899 17914 19.93 82.17 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : 19.93 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_4GKI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4gki-query.scw PDB file : Tito_Scwrl_4GKI.pdb: