Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSATTDRTQRPLLSKSKKLNSLHSKSSPALNFDESHSLSIKRRKVNTRDPAVYFVTIGS----------KRMLLRIYGIGCEQILDRDKELDWLSRLSRLNIGPSLLGTFDNGRFEEYLESTTLTWHDLRDPFISAQIASRLNQLHSIVDTFPPAEN--EPLEVWQNIDKWYRSLESEVLSTLKKNPVWAKMIEQSLDLSQLHKDIETCKSIL------NTLSTPTVFAHNDTQYGNILKIENTDELVVIDFEYAGYNPRGYDIANHFCEWMYDYHSSE-P-AKMNHKSYPTHKEQVRFLTAYDKHHV----------TELLREVELWKMACHLFWGLWGLVQASQS--EIDFDYFGYSLERLSVFKQELDAKATQIA 3C5I Chain:D ((14-357)) ------------PLYIKKICLEKVPEWNHF---TEDNLRVKQ-IL--LTNQLFEVGLKEETANNYNSIRTRVLFRIYGKHVDELYNTISEFEVYKTMSKYKIAPQLLNTFNGGRIEEWLYGDPLRIDDLKNPTILIGIANVLGKFHTLSRKRHLPEHWDRTPCIFKMMEKWKNQLFKYKNI-----EKYN------CDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTN--KCLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLDKVVPTPKLIDEILEAVEVQALGAHLLWGFWSIIRGYQTKSYNEFDFFLYAEQRLKMYDDQKE-------

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1344 11256 8.37 36.54 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : 8.37 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: